Generally - if you have to ask questions like this, then highly likely your work is not worth protecting. If only to avoid embarrassment when someone reads it out. Don't take this as a personal insult.
If you want a signature to find out who built upon your file - slightly change some irrelevant (unused) map.
If you want to leave a message for whoever reads the tune out - use free area in the binary, you can leave a friggin poem there.
If you want to completely protect the binary from being read out - that's generally not possible with ME7.
There are complicated ways to make read (or interpretation of the readout) a bit harder, but I won't really go too deep here. Let's just say that it involves low-level assembly programming and/or hardware mods to the ECU. I'm not a big fan of the latter.

There is a simple mod that prevents OBD readout by some (not all) tools, and it might be good enough for your purposes: http://nefariousmotorsports.com/forum/index.php?topic=1090.0

Thanks for your answer

Other think... is possible to merge the ecu map to the VIN number ? To only work only in that car?

Possible with custom assembly code.
But can be patched out by someone competent enough.

Oh very interesting... could you give me some advice... or show me the way to learn how to do it ?

No easy way to do it.
Learn to disassemble ME7 with IDA, learn assembly language, find EEPROM access functions, find where VIN is stored, implement some check. Some of this has been documented on this site, but still will take you a lot of time.
Or get someone to do that for you ($$). 

Time it's not a problem and i don't want someone that do this for me...i will not pay for this...in case i would pay someone to teach me how to do or guide me ... i need and want to learn ..not to have this done easily 

For example you have told me where to focus step by step in macro points

Thanks