Well I hope not.. They are calibrated towards fresh AIR at factory 21:0
You are misunderstanding...
We're not talking about free air calibration... We're taking about YOUR logs and the conversion factor you are using.
Most logging software reading AFR is on a gasoline scale. So unless you have modified the conversion factor to measure in the E85 AFR scale you are not interpreting the O2 readings correctly.
The ECU/O2 sensor doesn't read in anything but Lambda. The conversion is done outside by whatever software you are using to log with.
Similarly so, if you have an external wideband sensor, most are setup to read in Gasoline AFR scale. Running E85 will not magically change the scale, the wideband has to have an option for you to change the internal scaling.
In the end it is not necessary anyway. With E85 you simply target the same AFR as on a gasoline scale anyway. Lambda 1= 14.7 gasoline 0.8 lambda = 11.76 gasoline, etc, etc.